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Animals exposed to LCS exhibited impaired glucose tolerance, decreased insulin sensitivity, elevated fasting glucose, hyperinsulinemia, and impaired GLP-1 release — effects that sometimes occurred even in the absence of increased weight gain.
Effect sizefasting glucose levels 1.6-fold higher in ASP-exposed vs controls; 2.25- and 2.3-fold higher for males and females in ASP+MSG vs controls; insulin levels double those in plain-water control group in NNS-exposed mice
ComparatorPlain water controls or calorically-sweetened controls
Effect summarydecline; fasting glucose levels 1.6-fold higher in ASP-exposed vs controls; 2.25- and 2.3-fold higher for males and females in ASP+MSG vs controls; insulin levels double those in plain-water control group in NNS-exposed mice
Adverse eventsimpaired glucose tolerance, decreased insulin sensitivity, elevated fasting glucose, hyperinsulinemia, impaired GLP-1 release

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Source

PMC5045440
Low-calorie sweetener use and energy balance: Results from experimental studies in animals, and large-scale prospective studies in humans
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